Glucose Broth is recommended for study of dextrose fermentation where pH indicator is not desired. Waisbren, Carr and Dunnett used Glucose Broth for testing antibiotic sensitivity by the tube dilution method. This medium is also used to study glucose fermentation where pH indicator is not desired. It was developed to exclude the ingredients like beef extract that would contain small amount of carbohydrates. Thus the glucose fermentation studies can be performed more accurately using only pure 0.5% glucose as the source of carbohydrate.